Antisense Technology, Part A, General Methods, Methods of by John N. Abelson, Melvin I. Simon, M. Ian Phillips

By John N. Abelson, Melvin I. Simon, M. Ian Phillips

Antisense expertise is the power to control gene expression inside mammalian cells supplying robust experimental methods for the research of gene functionality and gene legislation. for instance, tools that inhibit gene expression let reports which probe the conventional functionality of a selected product inside a mobile. Such method can be utilized in lots of disciplines reminiscent of pharmacology, oncology, genetics, phone biology, developmental biology, molecular biology, biochemistry, and neurosciences. This quantity may be a really very important device in biomedical-oriented study. The significantly acclaimed laboratory general for greater than 40 years, Methods in Enzymology is among the such a lot hugely revered courses within the box of biochemistry. when you consider that 1955, every one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless correct today--truly a necessary booklet for researchers in all fields of lifestyles sciences.

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Johnston, D. J. Ecker, M. A. Zounes, W. F. Lima, and S. M. Freier, J. Biol. Chem. 267, 19954 (1992). 82 D. L. Sokol, X. Zhang, P. Lu, and A. M. Gewirtz, Proc. Natl. Acad. Sci. A. 95, 11538 (1998). 79 [1] PROGRESS IN ANTISENSE TECHNOLOGY 19 specific binding events, each of which may have different characteristics and effects. Nonspecific binding to a wide variety of proteins has been demonstrated. Exemplary of this type of binding is the interaction of phosphorothioate oligonucleotides with serum albumin.

Numerical Computer Methods (in preparation) Edited by LUDWIG BRAND AND MICHAEL L. JOHNSON VOLUME 322. Apoptosis (in preparation) Edited by JOHN REED xxxiii Section I General Methods This Page Intentionally Left Blank [1] PROGRESS IN ANTISENSE TECHNOLOGY 3 [1] Progress in Antisense Technology: The End of the Beginning By STANLEY T. CROOKE Introduction During the past decade, intense efforts to develop and exploit antisense technology have been mounted. With the recent FDA approval of Vitravene, the first drug based on antisense technology to be commercialized, the technology has achieved an important milestone.

Zounes, Science 257, 958 (1992). 8 S. T. Crooke, L. R. Grillone, A. Tendolkar, A. Garrett, M. J. Fratkin, J. Leeds, and W. H. Barr, Clin. Pharmacol. Ther. 56, 641 (1994). [1] PROGRESS IN ANTISENSE TECHNOLOGY 5 tides, uptake varies as a function of length, but not linearly. 8,9 Given the foregoing, it is obvious that conclusions about in vitro uptake must be made very carefully and generalizations are virtually impossible. Thus, before an oligonucleotide could be said to be inactive in vitro, it should be studied in several cell lines.

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