Bioengineering Applications of Carbon Nanostructures by Ado Jorio

By Ado Jorio

This e-book covers the advance of biotechnology in accordance with carbon nanostructures, with a spotlight on nanotubes, addressing additionally fullerenes and amorphous carbons. The publication is split into 7 chapters, addressing tissue engineering, genetic engineering and remedy, in addition to the environmental and well-being affects of carbon nanostructures.

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Materials 5:278–301 12. Vardharajula S, Ali SZ, Tiwari PM, Eroglu E, Vig K, Dennis VA, Singh SR (2012) Functionalized carbon nanotubes: biomedical applications. Int J Nanomedicine 7:5361–5374 13. Kam NW, Liu Z, Dai H (2005) Functionalization of carbon nanotubes via cleavable disulfide bonds for efficient intracellular delivery of siRNA and potent gene silencing. J Am Chem Soc 127:12492–12493 14. Liu Z, Winters M, Holodniy M, Dai H (2007) siRNA delivery into human T cells and primary cells with carbon-nanotube transporters.

1 h. Although this short half-life is not ideal for biomedical applications, there are many other parameters that regulate and improve the pharmacokinetics of CNTs, and among these we can cite the surface chemistry [26]. The pharmacokinetics of SWCNTs can be significantly altered by the type of suspending agent and functionalization method, with PEGylation being the most efficient method in improving the pharmacokinetics profile of CNTs. For instance, PEGylated-SWCNTs exhibit relatively long blood circulation times and high tumour accumulation [27].

However, higher InsP3R-II gene knockdown was achieved when cells were exposed to the complex for longer periods of time (24 and 48 h). In addition, cells incubated with CNTs alone did not show any alteration in InsP3R-II mRNA levels (Fig. 1). Likewise, Neagoe et al. in 2012 [21] used carboxylated Fig. 2 InsP3R-II siRNA:SWCNT complex reduces InsP3R-II expression levels in SKHep1 cells. a Representative confocal images showing InsP3R-II labeled cells. InsP3R-II staining was significantly reduced in SKHep1 cells transfected with InsP3R-II siRNA:CNT (middle and right panels) when compared to control cells (left panel).

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