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Additional info for Biomembranes Part A
The isolated mitochondria appear mainly in the condensed configuration (Fig. 5). They can readily be converted back to the orthodox configuration, 5~,'~6 which is more characteristic of mitochondria in the liver cell. The Golgi complex is sensitive to osmotic shock and handling (Fig. 7), but when properly prepared for electron microscopy (cf. Section V, Preparation of Samples for Electron Microscopy) shows remarkable preservation of structure (Fig. 8). Vesicles of rough microsomes correspond to the rough endoplasmic reticulum (Fig.
The cholesterol content of membranes varies significantly in the different fractions. Both plasma membranes ~ and Golgi complex contain high amounts of cholesterol. The cholesterol content of mitochondria and rough microsomes is quite low, by comparison (Table II). 5, R. W. Mahley, R. L. Hamilton, and V. S. LeQuire, 1. Lipid Res. 10, 433 (1969). ~8Subcellular fractions as isolated are frequently highly structured and contain nonmembranous components within their compartment. For analysis of membranes, such contents can be released from within the compartment, cf.
0 interface is somewhat cloudy. The indicated fractions are removed with a Pasteur pipette starting from the top of the tube. Chemistry The gross chemical composition of the granule and microsomal fractions is presented in Table II, where it can be compared to the composition of the whole homogenate. Comparable data from subceUular fractions of the guinea pig pancreas are given in Table I. The recovery of protein in the granule fraction is low but comparable to the value obtained by Greene. ~ The very low phospholipid:protein and RNA:protein ratios indicate that the final pellet is predominantly protein in both the guinea pig and bovine fractionation schemes.