Cell Culture for Biochemists by R.L.P. Adams (Eds.)

By R.L.P. Adams (Eds.)

This 2d revised variation equals the preferred 1st version in supplying a transparent and designated assessment of cellphone tradition. It provides info on: features of cultured cells; tradition vessels; glassware training and sterilisation innovations; subculturing; basic cells; phone tradition media; options; infection; the mobile cycle; phone synchronisation; use of radioactive isotopes in mobilephone tradition; mobile mutants and cellphone hybrids; viruses; and differentiation in phone cultures.

Reviews at the 1st edition:

``.. the e-book presents a superb perception into the way in which cellphone tradition concepts will be hired within the analytical examine of mobile biology.'' - developments in Biochemical Sciences

``It is easily written in a concise, easy-to-read type which stimulates the curiosity of the reader....'' - technological know-how Tools

``A worthy guide on ideas and practice.'' - Immunology at the present time

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1% prevented clumping and maintained viability. g. g. e. 2 and Eagle, 1959). 2. Density dependent regulation (contact inhibition) Primary cells will continue or start to divide in culture but exhibit contact inhibition of movement (Abercrombie and Heaysman, 1954). When two such cells approach one another the characteristic ruffling movements of the cell membrane stop in the area of contact. Primary cells therefore do not grow one on top of the other and, in general, cease to divide when a monolayer has been formed.

CULTURE VESSELS 41 Flasks can be readily given a gelatin coat as follows: a) Prepare a 1%gelatin solution in water and sterilise it by autoclaving at 1 5 p s i . for 15 min. 1% and add sufficient to a flask to cover the growth surface. c) Allow to sit at 4" for 1-2 h . d) Decant the gelatin solution and add the cell suspension in normal growth medium. 3. 2 X lo6 up to 2 x lo6. The containers may be glass or plastic. The plastic ware is obtained in sterile wraps from commercial suppliers and is specially prepared for use in cell culture (Fig.

Epithelial cells normally exhibit polarity (Chapter 2) and this is generally not obvious when they are attached to an impermeable plastic surface. Millipore and Costar each produce inserts for 6-well and 24-well tissue culture trays (Fig. 2b) which allow the cells to attach to a semi-permeable membrane and to contact the medium on both apical and basolateral surfaces. The medium inside the insert (apical surface) does not mix with the medium in the well outside the insert and Costar’s Transwell is designed to allow sampling of the two compartments with the insert in place.

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