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Additional resources for Cell Separation. Methods and Selected Applications, Volume 4
Instead of the single cell dispersion of vital dye-excluding cells which one obtains from normal liver, a mixture of cell clumps, few single cells, and largely undissociated tissue fragments is obtained. Attempts to mechanically disperse the perfused tissue into a single cell suspension lead to loss of cell viability. Because of these prob lems, it is often not possible to prepare oval cells and parenchymal cells from the same liver. Therefore, the perfused CDE liver tissue is next subjected to a second enzymatic digestion designed for isolation of either oval cells or parenchymal cells.
M. Steinman and R. J. ), pp. 7 1 - 9 7 . Rockefeller U n i v . Press, N e w York. , Katz, S. , Clement, L . , and Shevach, E . M. (1978). Immunologic functions of la-bearing epidermal Langerhans cells. J. Immunol. 121, 2005-2013. Stingl, L. A . , Sauder, D . N . , Pehamberger, H . , and Stingl, G. (1983). Mechanisms of U V - B induced impairment of the antigen-presenting capacity of murine epidermal cells. J. Immunol. 130, 1586-1591. Streilein, J. W . , Bergstresser, P. , and Tigelaar, R. E . (1985).
Cell. Immunol. 8 3 , 2 7 1 - 2 7 9 . + + 42 G. S C H U L E R E T A L . Fithian, E . , Fenoglio C , and Edelson, R. (1981). Reactivity of Langerhans cells with hybridoma antibody. Proc. Natl. Acad. Sci. A. 78, 2541-2544. Granstein, R. D . , L o w y , A . , and Greene, M. I. (1984). Epidermal antigen presenting cells in activation of suppression: Identification of a n e w functional type of ultraviolet radiationresistant epidermal cell. J. Immunol. 132, 5 6 3 - 5 6 5 . Huber, C , Merkenschlager, M .