Chemical Reagents for Protein Modification, Third Edition by Roger L. Lundblad

By Roger L. Lundblad

Revised and up to date, Chemical Reagents for Protein amendment, 3rd variation is an encyclopedic paintings describing the various methods to the site-specific amendment of proteins. greater than 2,000 references are mentioned describing the advance of a few of the reagents. This publication explores how using site-specific protein amendment pertains to a wide selection of biotechnology tactics and items, akin to protein microarrays, hydrogels, controlled-release prescription drugs, and biotherapeutics, and proteomics.With chapters targeting analyses of person amino acids, extra chapters hide chemical cross-linking and the chemical cleavage of peptide chains. there's huge connection with using site-specific chemical amendment in proteomics. the writer can pay specific consciousness to the final environmental elements that impression the reactivity of person amino acid residues. He offers suggestions for the characterization of changed proteins, together with mass spectrometry, and contains elevated insurance of spectroscopic options and crystallographic analyses. this can be by means of insurance of nitrosylation reactions and different non-enzymatic amendment procedures. particular reference is given to the differential labeling of protein practical teams with reagents classified with good isotopes. assets contain an inventory of present journals that submit site-specific chemical amendment reports, the main everyday reagents and their homes, and a touch checklist of reagent suppliers.Chemical Reagents for Protein amendment, third version offers the main often used equipment for the site-specific chemical amendment of proteins, suggestions for protein characterization, special laboratory facts for components that effect reactivity and reproducibility, and industry-specific assets.

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