By Claude Arthur Knight
In 1963, the 1st variation of Chemistry of Viruses used to be released as a contribution to the sequence on viruses backed through Protoplasmatologia. An objective of the 1st variation was once to check a few significant ideas and strategies of chemical virology in a concise demeanour and to accompany this evaluation with a compilation of pertinent references. It used to be expected that this workout will be worthy to the writer in his educating and learn and, confidently, will be priceless to readers in addition. The literature of virology has grown tremendously when you consider that then, and it really is much more pressing to have a succinct survey. additionally, few authors have tried to combine the findings referring to a number of the significant sessions of viruses (that is, animal, bacterial, and plant viruses) yet, fairly, have selected to collect huge monographs dealing intensive with evidence and fancies touching on particular teams of viruses. Such works are precious for pursuit of specific subject matters yet fail to yield a quick, built-in view of virology. the current variation of Chemistry of Viruses aspires to the sort of evaluate. a major try used to be made to deal concisely with each significant subject of chemical virology and to provide examples from diversified periods of viruses. a number of references are given to unique articles and overview papers in addition to to chose books.
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Extra resources for Chemistry of Viruses
And PIRIE 1938) that 1 per cent ·sodium dodecyl sulfate (SDS) disrupts TMV over a wide pH range. Later, when disruption by SDS wa's coupled with fractional precipitation with ammonium sulfate, it was shown (FRAENKEL-CONRAT and SIN GER 1954) that the protein ,and nucleic acid of TMV could he rather cleanly separated. ation. For example, trypsin is illhibited by anionic detergents suchas SDS (VISWANATHA et al. 1955), and TMV-protein prepared by treatment with SDS appears not to be satisfactory for structural studies dependent on a quantitative cleavage of protein by trypsin (FRAENKEL-CONRAT and RAMACHANDRAN 1959).
1his activity has been associated with the contracti'le tailprotein of the phagesand appears to differ distinctly from hostcell phosphatase adivity, especially with regard to effect of cations and pH 10 glycine buffier. Hence it ha's been concluded that there is a specific viral phosphatase buiIt into the particle. Another compelling case for the presence of a virus enzyme 1S the llcuraminidase found in influenza and other mYXJoviruses (see summary of GOTISCHALK 1957), aIthough cven here recent evidence caUs for a re-evaluation of the situation.
The suspension is centrifuged at 5000 g for 20 minutes. The supernatant is dialyzed against water to remove CaCI 2 • The dialyzed material contains the soluble protein but practically no RNA. 5. The Phenol Method (ANDERER 1959 a, Il) Disruption of viruses with phcnoi is the hasis for onc of the comlllonest methods for isolation of viral nucleic acids (sel' section on Methods for Virus Pruteins 39 Preparing Viral Nucleic Acids). In the preparation of nucleic acids the protein and other phenol-soluble components are usually discaDded in the phenolic layer.