Ciba Foundation Symposium - Control of Glycogen Metabolism by W. J. Whelan, Margaret P. Cameron(auth.)

By W. J. Whelan, Margaret P. Cameron(auth.)

Content:
Chapter 1 starting feedback (pages 1–6): F. G. Young
Chapter 2 constitution of Glycogen and Its Amylolytic Degradation (pages 7–28): Dexter French
Chapter three actual features of Undegraded Glycogen (pages 29–52): Stanley A. Orrell, Ernest Bueding and Magdalena Reissig
Chapter four Intestinal Hydrolysis and Absorption of Glycogen?Derived Oligosaccharides (pages 53–67): Arne Dahlqvist
Chapter five function of Uridine Diphosphate Glucose within the Synthesis of Glycogen (pages 68–86): Luis F. Leloir
Chapter 6 Branching Enzyme (pages 87–93): Joseph Larner
Chapter 7 The constitution of Phosphorylases (pages 94–106): Edmond H. Fischer, Michael M. Appleman and Edwin G. Krebs
Chapter eight The impression of Phosphorylase at the constitution of Glycogen (pages 107–122): Barbara Illingworth, David H. Brown and Carl F. Cori
Chapter nine The Enzymic Debranching of Glycogen and the position of Transferase (pages 123–138): M. Abdullah, Pamela M. Taylor and W. J. Whelan
Chapter 10 The function of Oligo?1,4>1,4?Glucan? Transferase and Amylo?1,6?Gluco?Sidase within the Debranching of Glycogen (pages 139–150): David H. Brown and Barbara Illingworth
Chapter eleven The Mechanism of motion of Amylo?I.6?Glucosidase (pages 151–175): H. G. Hers, W. Verhue and Monique Mathieu
Chapter 12 Animal Tissue ??Amylase and Its position within the metabolism of Glycogen (pages 176–192): E. L. Rosenfeld
Chapter thirteen basic dialogue on simple tactics of Glycogen Metabolism (pages 193–199):
Chapter 14 Interconversion Reactions of Muscle Phosphorylases B and A (pages 200–210): Edwin G. Krebs, Carmen Gonzalez, Jerome B. Posner, David S. Love, Gloria E. Bratvold and Edmond H. Fischer
Chapter 15 legislation of Glycolysis in Skeletal Muscle (pages 211–232): Ernst Helmreich, Simon Karpatkin and Carl F. Cori
Chapter sixteen The mobile place of Adenyl Cyclase and Adenosine?3,5??Phosphate in Escherichia coli (pages 233–246): Earl W. Sutherland, Peter Davoren and Richard Makman
Chapter 17 Biochemical results of Adrenaline on Intestinal delicate Muscle (pages 247–253): Ernest Bueding
Chapter 18 legislation of Glycogenolysis in Muscle: impact of Glucagon and Anoxia on Glycogenolysis within the Perfused Rat middle; influence of Adenine Nucleotides, Glucose 6?Phosphate and Inorganic Phosphate on Muscle Phosphorylase job. (pages 254–272): H. E. Morgan and A. Parmeggiani
Chapter 19 Insulin and the keep watch over of UDPG???Glucan Transglucosylase job (pages 273–293): J. Larner, M. Rosell?Perez, D. L. Friedman and J. W. Craig
Chapter 20 attainable oblique results of Adrenaline on Glycogen Metabolism (pages 294–300): P. J. Randle
Chapter 21 Hexokinase and Glucokinase (pages 301–304): A. Sols
Chapter 22 scientific Manifestations of Glycogen garage ailments (pages 305–320): Rudi Schmid
Chapter 23 Glycogen garage disorder, sort I (pages 321–335): D. J. Manners
Chapter 24 Glycogen garage illnesses, kinds III, IV, and VI (pages 336–353): Barbara Illingworth and David H. Brown
Chapter 25 Glycogen garage disorder, variety II (pages 354–365): H. G. Hers
Chapter 26 Glycogen garage disorder, sort V (pages 366–376): Joseph Larner
Chapter 27 Glycogen Synthetase Deficiency (pages 377–386): J. Spencer?Peet, G. M. Lewis and okay. M. Stewart
Chapter 28 reports of garage illness Glycogens (pages 387–415): Ernest Bueding, Stanley A. Orrell and James Sidbury

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Extra resources for Ciba Foundation Symposium - Control of Glycogen Metabolism

Example text

French: You mentioned (p. 36) a glycogen particle with a molecular weight of I 80 million, as measured by your counting procedure on the electron micrographs. Is that the molecular weight of the individual little particles shown in your figure or does it include the ones that look like dimers and tetramers and so forth? Onell: For simplicity I have called anything that will stand apart, that is not obviously a physicaljuxtaposition or aggregation on the electron micrographs, a molecule. The average of 180million refers to an overall weight average molecular weight ofthe compositesample.

A I - o ~ s I ~and , AI-oqg~z),the American Heart Association (62-G-21). and the Of€iceofNaval Research (Nonr-3417). 29 30 S. A . ORRELL, J R . , E. B U E D I N G A N D M . REISSIG In the course of studies of the comparative biochemistry of parasitic organisms, an attempt has been made to isolate glycogen from various tissues in quantitativeyields by means of a cold-water extraction procedure and to obtain some information about the properties of this material (Orrell and Bueding, 1958). , chloroform or a mixture of chloroform and octylalcohol, results in the precipitation of proteins at the interphase, while polysaccharides remain in the aqueous phase.

Hers: You showed (Table I) that your initial homogenization in chloroform and glycine buffer was carried out at low temperature. Why was that done ? Onell: In a number of samples we did not have quantitative recovery and enzymic losses were suspected. It was presumed that low temperature would reduce the activity of the enzymes for the few seconds necessary to mix the chloroform intimatelywith the material. The later chloroform shaking is done at room temperature because by then the glycogen preparation is relatively pure, and enzymic degradation is unlikely.

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