Colloidal Gold. Principles, Methods, and Applications by M. A. Hayat

By M. A. Hayat

Seeing that its advent in 1971, the improvement and alertness of colloidal gold as a marker in electron microscopy has been exceptional. This state-of-the artwork, multi-volume treatise offers researchers, technicians, academics, and scholars with the main accomplished assurance of the rules and technique of colloidal gold microscopy on hand today.
This common approach is acceptable to such a lot microscopical platforms together with optical microscopy, scanning, transmission and excessive voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold permits low and high solution reports, enzyme and nucleic acid labeling, learn of dynamic mobile tactics, and virus detection.

Topics lined in quantity 1 include:
* rules, equipment, and purposes of colloidal gold technique in cytochemistry and immunochemistry
* equipment for getting ready colloidal gold debris of other sizes
* Protein A-gold, protein G-gold, and lectin-gold techniques
* using resins and skinny cryosections
* a number of labeling

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Extra resources for Colloidal Gold. Principles, Methods, and Applications

Example text

8 9 , 653. G o o d m a n , S. L . , H o d g e s , G. M . , and Livingston, D . C. (1980). A review of the colloidal gold marker s y s t e m . Scanning Electron Microsc. 2 , 133. Gosselin, Ε . , Cate, C. C , Pettengill, O. , and S o r e n s o n , G. D . (1986). I m m u n o c y t o ­ chemistry: Its evolution and criteria for its application in the study of Epon-embedded cells and tissue. Am. J. Anat. 1 7 5 , 135. , van der D o n k , J. Α . , N o o r d z i j , Α . , van D a m , R. H . , and Vaerman, J.

J. Histochem. Cytochem. 3 1 , 987. , Taatjees, D . , L u c o c q , J. N . , and Paulson, J. C. (1985).

F o r further de­ tails, see H a n d l e y (1989). A n important surface p r o p e r t y of colloidal gold is its negative c h a r g e . In an a q u e o u s p h a s e , colloidal gold will stay in suspension by electro­ static repulsion. If electrolytes are a d d e d , the ion layers of the particles are c o m p r e s s e d and so the critical " c o h e s i o n " distance is r e d u c e d , thereby facilitating flocculation. It has b e e n recognized since 1901 (Zsig­ m o n d y , 1901) that gold particles can b e stabilized in solution by the addi­ tion of proteins.

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