High-Tech and Micropropagation I by Y. P. S. Bajaj (auth.), Professor Dr. Y. P. S. Bajaj (eds.)

By Y. P. S. Bajaj (auth.), Professor Dr. Y. P. S. Bajaj (eds.)

Presented here's one other vintage from this sequence and offers with basic features of micropropagation of vegetation for advertisement exploitation. It contains chapters on establishing a advertisement laboratory, meristem tradition, somatic embryogenesis, elements affecting micropropagation, disposable vessels, vitrification, acclimatization, induction of rooting, synthetic substrates, cryopreservation and synthetic seed. unique emphasis is given on smooth methods and constructing applied sciences equivalent to automation and bioreactors, robots in transplanting, synthetic intelligence, info administration and automated greenhouses for en masse advertisement construction of plants.

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4b). Setting Up a Commercial Micropropagation Laboratory 23 Fig. 4. a Petri dishes, note each plate is coded numerically. b Left Plastem container, note ridge which allows stacking. Right Kilner jar. c Clear food container in transparent stackable tray. d Heat-sealed double-wrapped container (see Fig. 3) In this laboratory, we have exploited the concept of "biopackaging", in which plastic food quality containers are heat-sealed with membranes which have differential permeability to oxygen, carbon dioxide, ethylene and water vapour (Cassells 1987) (Fig.

Plants with loosely covered buds, such as potato, carnation, and impatients, should be sprayed with a systemic disinfectant. 1 % Streptomycin sulfate has been used successfully. In addition, it is beneficial to spray stem sections from trees and shrubs having winter buds. Microorganisms are abundant between the bud scales because of the stem's long period of exposure to open air and rainfall. Some segments should be retained on the mother plant and sprayed with systemic disinfectant at the 10th and 5th day before excision.

0 I:':',' '. I. Diagram of a shoot meristem containing the apical meristem (apical dome) and the meristematic subapical region with attached leaf primordia 34 P'-l. Wang and A. 1 Equipment In our laboratory at Geo. J. , the following supplies have been used: 1. 2. 3. 4. 5. 6. 7. 8. 9. 2 Plant Material Preparation Actively growing shoot tips are recommended for meristem culture. When shoots are in an active growth phase, the pathogen concentration tends to be reduced and the growth potential after dissection is greater.

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